This study provides a basis for further research and will aid the conservation of this species. kwangsiensis based on the ML tree (Figure 1). The topology showed that 11 species of the genus Aristolochia occur in the same clade. Based on the substitution GTR + I + G model, the maximum-likelihood (ML) phylogenetic tree was produced using RAxML-HPC2 on XSEDE ( ) with 1,000 bootstrap replicates (Stamatakis et al. The best model was generated using jModelTest2 on XSEDE (, Miller et al. Sequences were aligned using the MAFFT alignment tool on Geneious Prime v.2021.2.2 (Biomatters, Auckland, New Zealand, ). The sequences used for alignment are available in the GenBank database, including A. Three species of the genus Piper were used as outgroups. To explore the phylogenetic relationship, a phylogenetic tree was generated using five species of the subgenus Aristolochia and six species of the subgenus Siphisia. The trnK-UUU gene included the longest intron at 2644 bp. Among these genes, 15 contained one intron and three contained two introns. In total, 113 genes were annotated, including 79 protein-coding genes, four rRNAs, and 30 tRNAs. The complete plastome genome was found to be 159,764 bp in length with a GC content of 38.8%, including a large single-copy (LSC) of 89,134 bp and a small single-copy (SSC) of 19,306 bp separated by a pair of inverted repeats (IRs) of 25,662 bp. The complete plastome genome was submitted to GenBank under the accession No.
#Geneious prime citation manual#
2019) followed by a manual check with Geneious Prime v.2021.2.2 (Biomatters, Auckland, New Zealand, ) to obtain the final annotated data. Annotation was conducted using PGA (Qu et al. After filtering low quality reads, a total of 2.38 Gb of clean data with Q30 (92.02%) were obtained and assembled with GetOrganelle (Jin et al. The DNA library was sequenced using an Illumina NovaSeq 6000 platform with 150 bp pair-end read lengths at Novogene Co. Fresh leaves were sampled for total genomic DNA extraction using a modified CTAB method (Doyle et al. A specimen was deposited at SCAUB (, Rongjing Zhang, under the voucher number Rong-Jing Zhang 32021. We collected several individuals from Mt. hainanensis for the first time, and aimed to provide basic genetic information harbored in the chloroplast and unveil the phylogenetic position of A. In the current study, we revealed the chloroplast genome of A. In recent years, many chloroplast genomes of other species in the genus Aristolochia have been reported, including A. The chloroplast genome is crucial for the taxonomic classification and phylogenetic analysis of land plants (Moore et al. hainanensis has been listed as a Threatened Species with vulnerable (VU) status on the IUCN Red List ( ).Ĭhloroplasts are important organelles of green plants with roles in photosynthesis and nitrogen fixation (Moore et al. In recent years, with the degradation and loss of their natural growth environment and human harvesting and cutting, A. 1922, which is a traditional Chinese medicinal plant mainly distributed in the Wuzhishan Mountain region of Hainan Island. In the current study, we focused on the species Aristolochia hainanensis Merr. The Aristolochiaceae are composed of approximately 550 species, most of which belong to a large group in the genus Aristolochia, which includes 450 species (Bliss et al.
0 kommentar(er)
